As shown in Figure 1D, cell growth was arrested during the next 3 days in the absence of citrate, but restarted after the 6th day and cells progressively colonized the flask in about two weeks. A slow colonization of the flasks by cells previously exposed to the higher concentration of citrate (20 mM) was also observed, although to a lower extent (data not shown).
Therefore, the possibility of impeding this proliferation by administering cisplatin (over 2 hours) at the end of citrate exposure (10 mM) was studied. A cisplatin dose which did not present any cytotoxic effect per se, but was only cytostatic, was chosen. Indeed, the dose of 5 μg/mL (C5) demonstrated only a transient slowing down of the proliferation of MSTO-211H cells (Figure 2A and 2B). Thus, cisplatin or citrate alone appeared unable to prevent cell proliferation. In contrast, when cisplatin was associated with citrate, massive apoptosis occurred without any recovery of cell growth, even after 14 days (Figure 2C).
http://ar.iiarjournals.org/content/29/4/1249.long
