Persephone
Jedi Master
I don't know if it is the same but Dr Vélot talks about recombinant DNA virus vaccines :I'm not familiar with recombinant DNA technology and how that differs to a DNA Vaccine e.g. that of Oxford.
Not sure if anyone knows?
the genetic material that we want introduce into cells - either RNA or DNA cannot enter alone into cells, we need to use a carrier, which is called a vector.
In the case of recombinant DNA vaccine the vector is not a lipid nanoparticle but a virus, not the same virus of course (SARS-COV2) but another virus that they use for its natural properties : being able to inject genetic material into cells. This recombinant virus is an adenovirus ( a DNA virus responsable of a mild flu state) which has been previously desarmes, rendered inoperative by eliminating some of its genetic material responsable for its virulence. The genetic material eliminated is replaced by the part of the SARS-COV2 genetic material that holds tha manufacturing code of the Spike protein.
This means that this adenovirus will deliver in our cells hybrid genetic matrial which is part of itw own genetic material and part of the genetic material of SARS-COV2.
As SARS-COV2 is a RNA virus and the adenovirus vector is a DNA virus and that we cannot insert RNA in DNA, the SARS-COV2 RNA has been previously converted into DNA in the laboratory thanks to a viral enzyme, via reversed transcriptase. Then only the required portion is recovered and inserted in the DNA of the virus used as vector.
This kind of vaccines inject DNA in our cells which, unlike RNA (at least not directly), can be integrated into our DNA. The problem is that thay don't have any control over the place where it can insert...