The highest value that I have seen in studies is 28 mg for American Spirit. Most other cigarettes are much lower, some below 10 mg.
Yes, it's very hard to know the exact content.
I usually guess going by what the Cs said (but taking into account that it may not be precise, since the tobacco is different).
cassiopaeatranscripts.org
Q: What other things does nicotine do?
A: Raises defences.
Q: What kind?
A: Immunological.
Q: Anything else?
A: Excites neurotransmitters. You require less sleep.
Q: Is this true for everyone?
A: No.
Q: (A) How much nicotine is necessary?
A: 100 mg per day.
Q: (A) Can it be in pill form?
A: Cigarettes infuse it to brain tissues most effectively.
Q: Well, I am certainly relieved! Now I know that I do not have a 'smoking demon' possessing me! I was really getting worried!
A: Those who fit this profile find it nearly impossible to "quit" completely.
Q: So, there are people who are actually benefitted by smoking?
A: Genetics will offer proof of this.
Q: You mean that one can see changes in DNA before and after smoking?
A: Close
Q: Is this also true for Frank?
A: Yes.
Q: Why us?
A: It is simply part of your "profiles."
Q: Is that also why I have been having more stomach problems than usual?
A: Yes.
Q: Well, I always noticed that smoking could settle my stomach. It also stimulated my bowels... (F) Yes.
A: (A) What is serious here is the number. How many milligrams... (L) How many cigarettes does the number translate into?
A: 20.
So, an average of 5mg per cigarette.
I used to smoke between 15 and 25 rollies per day. So, between 75mg and 125 mg of nicotine.
Our vaping liquid is at 10mg/ml. And I use roughly 5m per day. I.e. 50 mg. When I feel the need for more (or just for pleasure), I will smoke 1-3 cigarettes a day. That brings the total to about 55-65 mg. But it's not exact, because in those days, I can vape less too.
That's very interesting. Did the total daily amount of nicotine stay the same or was it reduced as well? Because the question is also whether one can achieve the same improvement simply by reducing the daily amount of smoked cigarettes to a certain degree.
I hope the calculation above helps you with that. But I think it is very different, and it depends on what effects you are talking about. For the vocal chords, for example, nicotine has probably nothing to do with it, and it is the by-products of combustion that have the bad effect. For mental concentration, I think it might depend on what you brain really needs: stronger boosts less times per day (smoking), or smaller boosts more regularly? (vaping). And for HRV, as far as I understand there are several factors involved, such as the type of exercise you do, your quality of sleep, etc. But if anything, my guess would be that the lower HRV in smokers also has to do with the byproducts of combustion, not nicotine itself.
Here are some articles, but I haven't read them thoroughly. They seem to be saying that the increase in HRV after smoking cessation is kind of temporary. Other say it definitely is an issue for smokers.
Evidence from both laboratory and observational studies suggests that acute and chronic smoking leads to reduced high frequency heart rate variability (HF-HRV), a measure of cardiac vagal regulation. We utilized ecological momentary assessment (EMA) ...
pmc.ncbi.nlm.nih.gov
Background: Cigarette smoking has been associated with increased activity of the sympathetic nervous system. In this study, we investigated cardiac autonomic function in heavy smokers and nonsmoker controls by analysis of heart rate variability ...
pmc.ncbi.nlm.nih.gov