Coronavirus Pandemic: Apocalypse Now! Or exaggerated scare story?

Another study on the psychological effects of lockdowns featured in The Guardian today.

www.theguardian.com

Pandemic triggered ‘second midlife crisis’ among over-50s, study finds

Women hit worst by mental health strains, with more domestic and caring duties amid Covid lockdowns
www.theguardian.com www.theguardian.com

The distress caused was apparently worse than 'mid-life crisis' anxiety and was significantly worse for women.

A study has found that just before the second national lockdown began, those born in Britain in 1958 and 1970 were experiencing higher levels of psychological distress, on average, than they had ever experienced in adulthood before, surpassing previous peaks in their early 40s.
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This peak of anxiety during the first year of the pandemic was higher for women than men across all age groups, widening the already substantial gender inequalities in mental health that existed before the pandemic.

“Most of the studies we analysed propose that this is due to a totally different share of the domestic and caring responsibilities during lockdown,” said Dr Darío Moreno-Agostino, lead author of the report.

“An increase in gender-based violence and abuse, as well as additional financial constraints, are also suggested because there’s evidence showing that women were in more precarious situations than men: for instance, from the work point of view, more care workers – typically women – were exposed to more difficulties than other professions,” he added.
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“There is the question of resilience, but we know that anxiety and depression are the top leading causes of disease worldwide,” said Moreno-Agostino. “Those mental health problems are closely linked to numerous physical health problems – right up to and including increased morbidity.

“The fact that we are observing an unexpected new peak of the very mental health problems that can lead to these long-term trajectories is deeply concerning,” he added.
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Some comments from Lord Sumption to the Telegraph. Lord Sumption was outspoken in his opposition to lockdowns and most other plandemic measures over the last couple of years.


Not really news to anyone here but his main point is that the BBC followed the government line on lockdowns due to threats to licence fee funding:

"The BBC, which is probably the most influential single media organ, was consistently pro-lockdown. It was, of course, under threat to its financial model from the government and it therefore wanted to show that it was 'good boys'."
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"Within the BBC, I know for certain that there were a variety of opinions, but the one which they chose to concentrate on was that the lockdown was a great idea, that people who were sceptical were being anti-social, and there was very little to show that there was an alternative view held by responsible people."
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He also comments on the UK Covid Enquiry and it's delays. He essentially points out that once again the Swedes get things right (and I do not know how true this really is in Sweden) by using a body of actual experts to assess the relevant evidence of the effectiveness of measures, rather than just letting anyone have a say with whatever level of knowledge/expertise they may or may not have:

"The basic problem is that for about 50 years now, public inquiries have become increasingly legalised and judicialised, as they are regarded as occasions for everyone to have their say,, for victims to achieve what is sometimes called closure, rather than to ascertain the facts. You end up with endless cross-examination."

"A much more sensible system, which was, for example, adopted by the Swedes, would be to have a body of experts who will use such evidence as they think is necessary, rather than what anyone else might think necessary, to arrive at the facts and form a judgement. That is a model of what we should be doing, instead of having this kind of quasi-forensic process which lasts a very long time."
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A good news. (?)

www.infobae.com

El Gobierno eliminó los registros de datos COVID de la aplicación Cuidar

Según se informó en el Boletín Oficial es por el fin de la emergencia sanitaria y para resguardar los datos personales de la población. Se dio de baja la plataforma y las 12 base de datos que contenía
www.infobae.com www.infobae.com

Government removed COVID data records from the Cuidar application

The National Government (Argentina) eliminated the databases linked to the Cuidar application used for the registration of vaccines against COVID-19 and other permits linked to the pandemic restrictions. According to Provision No. 2/2023 published in the Official Gazette, the reason is the end of the public health emergency due to the coronavirus pandemic and, in addition, "to safeguard the personal data of the citizens".

The decision was taken within the framework of Law No. 25,326 on Personal Data Protection, which establishes in Article 22 that the rules on the creation, modification or deletion of files, records or data banks belonging to public agencies must be made by means of a general provision published in the Official Gazette of the Nation and determines the destruction of those data that are no longer necessary or relevant to the purposes for which they were collected.
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Corona Investigative Committee, Berlin
Session 150 • “Per Aspera ad astra”

So, I am still listening to parts of the latest Corona Session (which by the way, as of now and for unknown reasons, isn’t available at Odysee yet, and only partially at gettr).

But it is one of those important, really heavy ones, where you afterwards sit there, with a jaw dropped down. Can it get any worse ? Oh yes, apparently it can. Pandora’s box has been opened and it is even worse than we thought regarding the Covid-19 jabs and the overall mRNA platform - since more important evidence has come into the light in the last month.

Since it is a complex subject and i am in the middle of my work at the subway (during a break), i’ll write about it when i am off duty from my work: on monday or Tuesday i’ll try to capture the details, watching the illustrations (which i have not seen yet as i only listened to part of the session) and try to form words about the essence in what they revealed/talked about (Dr Michael Palmer, Prof Dr Ulrike Kämmerer, Wolfgang Wodarg)

Short content
It is about the contaminants in the jabs which also contain a high amount of DNA, and not just the desired mRNA. The European EMA set a theshold of acceptance of DNA presence in jabs to max 1/3000th part (without motivating where that number comes from or why it was set there). But it is very low.

So, the early jabs contained in practice an already a high amount of 1.5% DNA. But the newer bivalent jabs, a whopping amount of 25% DNA. And here Pandora’s box starts to unfold into fully uncontrolled territory regarding illnesses and complications for human health, uncontrolled alterations of the human genome - as the DNA from bacterium integrates with the human genome and even the primary human DNA in sperm/eggs.

If we earlier mainly suspected and then also got confirmed that RNA indeed can integrate into cell DNA, through reversed transcripease - but didn’t understand nor know how absurdly high amounts of DNA material was left in the jabs.

Now those are confirmed to be present in such large amounts, that this perhaps is the major culprit - the cause - of alterations of the human cell genome, causing all kinds of (genetic based/caused) diseases (parts of which we still have not seen yet fully unfolding, due to that some take time to unfold, in children from iabbed parents, as well jabbed children later, when they grow up, etc).

Again, i am not sure if i got it right in detail, but it was really daunting after having listened to the summary of details, the consequences from the latest findings presented at the Corona Committee

But - it is a very important session i think is important to listen to (i hope they get the english and german videos published at Odysee).
 
XPan said:
Corona Investigative Committee, Berlin
Session 150 • “Per Aspera ad astra”
...
(Dr Michael Palmer, Prof Dr Ulrike Kämmerer, Wolfgang Wodarg) --- It is about the contaminants in the jabs which also contain a high amount of DNA, and not just the desired mRNA. The European EMA set a theshold of acceptance of DNA presence in jabs to max 1/3000th part (without motivating where that number comes from or why it was set there). But it is very low. --- So, the early jabs contained in practice an already a high amount of 1.5% DNA. But the newer bivalent jabs, a whopping amount of 25% DNA. (correction: 20-35% DNA)
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IMAGE-2023-04-08-19-19-34.jpg

I just stumbled across a substack, realizing one of its contents this was the very content that was discussed at the latest Corona Investigative Committee, No 150 "Per aspera ad astra" yesterday.

I will not re-tell the full content in my own words (which often gets quite quirky and roundabout - but i may fill in more content a couple days later, of what Dr Wolfgang Wodarg and Prof Dr Ulrike Kämmerer added in the session, e.g. about the uptake of intact, replicating-capable bacterial DNA plasmids (present in the "vaccines") via our intestines, producing long lasting spike proteins as well creating antibiotic resistance :rolleyes: - that a thing like that, makes your head spin.

A little taste of it: Note the in red written text: It was new to Dr Kämmer, that B lymphocytes have an ancestral property of taking up plasmid DNA. Such implication are staggering.

_-2023-04-08-at-19-38-52-jpg.73217


Note: The english version of the latest Corona Session has still not been published yet (they did have a lot of technical difficulties and things were delayed - so that might have played into it). Only the german version is out.

Only in German, as of now
odysee.com

Sitzung 150: Per aspera ad astra

English version: https://odysee.com/s150en
odysee.com odysee.com
starting at 3h 54min

Nevertheless, below you get the professional version of what "Pandora's box" is about, e.g. the extremely high content of bacterial DNA/plasmids in the latest bivalent mRNA jabs, and beyond the already many known paths of illnesses - we face additionally new implications, due to the integration into chromosomes. I also wonder what the additional of antibiotics getting snuck in, might do to all of this...


COVID-19 mRNA vaccines contain excessive quantities of bacterial DNA
Evidence and implications.
by Dr Michael Palmer [immunologist] and Jonathan Gilthorpe, PhD + (Kevin McKernan and Prof Dr Ulrike Kämmerer added corrections and discussions)

doctors4covidethics.org

COVID-19 mRNA vaccines contain excessive quantities of bacterial DNA: evidence and implications

Recent studies by Kevin McKernan, a leading expert in sequencing methods for DNA and RNA, have revealed that batches of the modified mRNA vaccines produced by both Pfizer and Moderna contain a high proportion of contaminating bacterial DNA. This document summarizes the evidence of that DNA...
doctors4covidethics.org doctors4covidethics.org
(a pdf you find in the end of this entry)

Abstract:
Recent studies by Kevin McKernan, a leading expert in sequencing methods for DNA and RNA, have revealed that batches of the modified mRNA vaccines produced by both Pfizer and Moderna contain a high proportion of contaminating bacterial DNA. In all, the DNA accounts for up to 20-35% of the nucleic acids contained in each of the vaccine batches. These alarmingly high concentrations far exceed the levels deemed safe by standard-setting organizations such as the European Medicines Agency (EMA). This document summarizes the evidence of that DNA contamination and discusses what possible health risks it implies to the recipients of the vaccines.

Proinflammatory effect of bacterial DNA:
The human innate immune system reacts with inflammation to various bacterial macromolecules, including DNA. The large amounts of DNA present in the vaccines must be assumed to contribute to inflammation near the injection site, and potentially also elsewhere in the body.

Genomic integration in germline cells.
Oocytes can be transfected in vivo at certain stages of maturation, and so can sperm-producing cells within the testes In the latter case, the offspring of the animals subjected to such treatment were shown to be transgenic. It can therefore not be ruled out that persons injected with mRNA vaccines that also contain DNA will subsequently give rise to transgenic children. DNA insertion into germline cells might also interfere with early intrauterine development and thereby induce miscarriages or malformations.

Conclusion:
The presence of contaminating plasmid DNA in Pfizer’s and Moderna’s mRNA vaccines entails severe health risks, in addition to those which were already known and understood. Preeminent among these risks are the prolonged expression of spike protein, which may lead to correspondingly prolonged and more destructive autoimmune-like inflammation, and the induction of malignant disease after chromosomal integration of the plasmid DNA. Furthermore, the sheer scale of the contamination proves conclusively that the manufacturers have not mastered or properly implemented the designed production processes. Each of these issues alone would be reason enough to demand the immediate withdrawal of these vaccines.


FULL TEXT - for reference and archive
Recent studies by Kevin McKernan, a leading expert in sequencing methods for DNA and RNA, have revealed that batches of the modified mRNA vaccines produced by both Pfizer and Moderna contain a high proportion of contaminating bacterial DNA. In all, the DNA accounts for up to 20-35% of the nucleic acids contained in each of the vaccine batches. These alarmingly high concentrations far exceed the levels deemed safe by standard-setting organizations such as the European Medicines Agency (EMA). This document summarizes the evidence of that DNA contamination and discusses what possible health risks it implies to the recipients of the vaccines.

1. The role of DNA in the manufacture of mRNA vaccines​

1.1. General background​

Most readers will be aware that

  1. the synthetic RNAs contained in the COVID-19 mRNA vaccines encode the SARS-CoV-2 spike protein;
  2. in living mammalian cells, the instructions for building a given protein molecule are stored as a gene within the DNA inside the nucleus;
  3. to build a given protein molecule, the cell first transcribes its gene into RNA and modifies the two ends of this molecule to form messenger RNA (mRNA). The mRNA is then transported from the nucleus to the cytoplasm, where it induces the cell’s protein factories—the ribosomes—to translate the mRNA’s nucleotide sequence into the corresponding amino acid sequence and assemble the protein.

1.2. Steps in the manufacture of mRNA vaccines​

Since the spike protein is a large molecule, so is the mRNA which encodes it. The total chemical synthesis of large mRNA molecules is not practical at scale. Therefore, in order to obtain the mRNA molecule encoding spike, the process by which cells produce their own mRNAs is mimicked in vitro. This involves the following steps:

  1. A DNA copy of the gene for the spike protein is inserted into a bacterial plasmid. This is a ring-shaped, double-stranded DNA molecule which can exist in a bacterial cell independently of the cell’s own chromosomal DNA, and which can also be copied and passed on to both daughter cells when that cell divides.

  2. The recombinant (artificial) plasmid carrying the spike protein gene is introduced into a cell of the bacterial species Escherichia coli (E. coli ). Since E. coli cells divide very rapidly, this one cell can within a short time be grown up to a very large number of cells. Each of these progeny cells will contain their own inherited copies of the plasmid, and therefore of the spike protein gene.While there is a certain chance of the plasmid being lost from some of the offspring during successive cell divisions, we can enforce its maintenance by giving it a selectable marker, which ensures that only those cells which retain the plasmid will survive. With the plasmids used by both Pfizer and Moderna, this selection marker is a gene which endows the host cells with resistance to the antibiotic kanamycin. To apply the selection, the bacteria are simply grown in the presence of kanamycin.

  3. After growing up a sufficient number of bacterial cells in a nutrient broth containing kanamycin, these cells are broken up and the plasmid DNA is purified from the other bacterial cell components.

  4. The ring-shaped plasmid molecules are converted to linear form using a restriction enzyme, which cleaves both strands of the DNA molecule at a specific, unique site which is located downstream of the spike protein gene. This step is needed to prevent the formation of RNA molecules that are overly long and might have undesired effects in vivo.The linearized DNA molecules can be purified from remaining circular ones, but in what manner and how efficiently this may be done in the production of Pfizer’s and Moderna’s vaccines is not public knowledge.

  5. An RNA polymerase is used, in the presence of the necessary nucleoside building blocks and cofactors, to copy the spike protein gene from the DNA version on the linearized plasmid into the mRNA version. Both Pfizer and Moderna employ the T7 RNA polymerase, which is derived from the eponymous bacteriophage. This enzyme binds to a cognate promotersequence likewise derived from T7 that has been engineered into the plasmid upstream of the gene for the spike protein. This interaction between polymerase and promoter initiates the transcription.At this stage, the synthetic nucleoside N-methyl-pseudouridine (mψU) is incorporated into the artificial RNA instead of the natural uridine nucleoside. When delivered in the form of a vaccine, RNA modified in this manner is less stimulatory to the innate immune system than is RNA containing the natural uridine. It is also more efficiently translated into protein, and under certain conditions more resistant to degradation [1]. Both Pfizer’s and Moderna’s mRNA vaccines contain mψU instead of uridine.

  6. The two ends of each RNA molecule are coupled enzymatically to certain moieties that are also found at these positions within natural mammalian mRNAs, and which enhance its biological activity and stability in vivo.
This steps provide a functional mRNA which is capable of instructing the cells’ ribosomes to produce the spike protein. However, at this stage the product is not yet pure—all of the bacterially derived template DNA is still present. The latter should not be included in the final drug product, because it poses health risks to the recipients (see Section 4). To get rid of this DNA, another enzyme called DNase is added. This should break up the DNA into smaller fragments, which can then be removed from the much larger RNA molecules by filtration and other purification techniques. In the final step, the mRNA is combined with a lipid mixture in order to package it into lipid nanoparticles (LNPs), which induce human cells to take up the mRNA molecule and to make the spike protein.

2. What did we know previously about the DNA contamination problem?​

In a nutshell, very little. The FDA’s assessment reports on both vaccines [2,3] do not mention the issue at all. The European Medicines Agency (EMA) assessment report on the Pfizer vaccine mentions that “The robustness of the DNase digestion step is not considered comprehensively demonstrated” [4, p. 17]. Similar language is used in the EMA report on the Moderna vaccine [5, p. 19f]. However, based on this sparse information alone, it is impossible to say whether the problem was considered serious, and what remedies were required by the regulator, if any.

3. Independent evidence about DNA contamination of mRNA products​

As of April 3, 2023, Kevin McKernan has described his recent findings in three articles on his Substack site [6–8]. The experiments described in the first two reports were carried out on samples of newly introduced “bivalent” vaccines from Pfizer and Moderna. These preparations resemble the previous “monovalent” ones in their chemical composition, i.e. they should contain highly pure mRNA, complexed with a mixture of lipid (fat-like) molecules into mRNA /lipid nanoparticles. The only difference between the two varieties is that the bivalent vaccines contain a mixture of two mRNAs encoding two antigenic variants of the spike protein. This has no bearing on the technical problem of DNA contamination as such. We note, however, that the extent of DNA contamination may vary between production batches, and that only a small number of batches has so far been characterized in this regard.

3.1. McKernan’s first report​

In an initial study [6], McKernan characterized both the RNA and the DNA contained in the mRNA vaccines.

3.1.1. Extraction and direct characterization of nucleic acids from the vaccines​

The first step consisted in stripping away the lipids in order to obtain the pure nucleic acids. The solvent-based method that he used does not discriminate between DNA and RNA—if both are present, both will be recovered. The extracted nucleic acids were separated according to size. This revealed not only the expected regular, full-length spike mRNA species, but also smaller fragments, which had been noted previously both by the regulators and in work published by one of the manufacturers [9]. More surprisingly, RNA species larger than the full-length mRNA were also found. These species remain uncharacterized.

3.1.2. Amplification of the extracted nucleic acids​

As a preparatory step for determining the exact nucleotide sequences of the extracted nucleic acids, they were amplified by PCR methods. In the case of the RNA, PCR was preceded by reverse transcription into DNA using a dedicated enzyme (reverse transcriptase). Since this study’s primary goal was to study the RNA rather than the DNA, this amplification step was biased against DNA through the addition of actinomycin D, which under the given experimental conditions selectively inhibits DNA synthesis. Accordingly, relatively low amounts of DNA were recovered in the amplified sample. Nevertheless, in case of the Pfizer vaccine, the amount of DNA determined to be present already exceeded EMA’s arbitrarily decided limit for the maximal permissible proportion of DNA per RNA.

3.1.3. DNA sequencing results​

With both Pfizer’s and Moderna’s products, DNA sequences of complete DNA plasmids were obtained, although some ambiguity remained in the case of the Moderna plasmids. The features of the plasmid sequences will therefore be discussed in connection with McKernan’s second study, which used more and more pure DNA for sequencing and therefore provided more reliable results.

3.2. McKernan’s second report​

The second study [7] focused on quantifying and characterizing the DNA contamination that was qualitatively detected in the first one.

3.2.1. Plasmid DNA contained in the mRNA vaccines is competent to propagate in bacterial cells​

In the first experiment, it was determined whether the plasmid DNA whose presence had been inferred from the previous sequencing results is indeed biologically functional, to the extent that it can be introduced into and persist within bacterial cells. To this end, nucleic acids were again extracted from the vaccine samples. These nucleic acids were mixed with a suspension of E. coli cells that had been rendered competent for DNA uptake.

After inducing these cells to take up the DNA and giving them some time to recover, they were spread onto Petri dishes filled with solidified growth medium containing kanamycin. As noted earlier, kanamycin will kill any E. coli cells that do not contain a resistance gene to it. Therefore, the observed growth of bacterial colonies on those Petri dishes confirmed that some cells had indeed acquired resistance to kanamycin by taking up and propagating the plasmids. This was observed with both the Pfizer and the Moderna vaccine samples.

In this context, we should note that only circular plasmid molecules, but not linearized ones, can be efficiently introduced into bacterial cells. The success of this experiment therefore suggests that some of the plasmid molecules had escaped the linearization step (step 4 in Section 1.2) and made it all the way through the production process in the circular form which exists in bacterial cells. On the other hand, since the number of bacterial colonies observed in this experiment was not high, it is likely that most of the DNA had indeed been linearized. Because the biological hazards of foreign DNA within our own body may vary depending on whether it is linear or circular, the likely presence of both forms in the vaccines is worth noting. The exact proportions of circular and linear DNA in the mixtures remain to be determined.

3.2.2. The abundance of contaminating DNA​

The second major finding of this study is the quantitation by PCR of both DNA and mRNA contained in the vaccine samples. As you may be aware, in a PCR reaction, a chosen segment of a nucleic acid sequence is reduplicated by enzymatic synthesis in several successive reaction cycles. From the number of cycles (or doublings) necessary to reach a certain threshold concentration, we can calculate how many copies of the target sequence were present at the outset.

In these experiments, the chosen experimental format was multiplex PCR, i.e. two target sequences were amplified in a single reaction mixture. One of these targets was within the spike protein gene, and it thus should be present both on the plasmid DNA molecules and on the spike mRNA molecules transcribed from them. In order to include the mRNA molecules in this amplification, PCR was again preceded by reverse transcription.

The other target sequence was within the kanamycin resistance gene, which should be present only on the plasmid DNA. By comparing the number of cycles required for each of the two targets to cross the threshold, it was determined that up to 35% of the total nucleic acid contained in the vaccines is in fact DNA. For comparison, the EMA has stipulated that DNA should not amount to more than 0.033% of the total nucleic acids.

3.2.3. Determination of plasmid DNA sequences​

The plasmids that had originally been contained in the vaccines and then been introduced into bacterial cells (see Section 3.2.1) were again isolated from those bacterial cultures, and their complete DNA sequences were determined. Such sequences were provided in full in McKernan’s first study [6], but he indicated that he was still working on corroborating and refining the sequencing data. Meanwhile, the functional features of the plasmid DNA found in the Pfizer vaccine samples are shown in Figure 1. They will be discussed in connection with the risk assessment.


pfizer-plasmid-map-9417f.png
Figure 1:
Map of the plasmid DNA contained in one of the bivalent Pfizer vaccine vials. Functional features are inferred from the experimentally determined DNA sequence. The gene encoding the spike protein (red), whose transcription is driven by the T7 promoter, accounts for about half of the total DNA sequence. The “NeoR/KanR” gene (light green) encodes a protein that will render bacterial cells resistant to kanamycin or neomycin, or human cells resistant to the related antibiotic G418.

The yellow sequence labelled “ori” is the bacterial origin of replication; it will cause copies of the plasmid to be made within the bacterial cell.

The SV40-derived elements at the top left can induce expression of G418 resistance in human cells, and they also include an origin of replication that may cause propagation of the plasmid in human cells. They are absent in Moderna’s plasmids, which are otherwise similar to Pfizer’s. See text for further details. Figure adapted from [7].


3.3. McKernan’s third report​

In his thus far latest report, McKernan examined eight vials of an earlier batch of the Pfizer vaccine using the quantitative PCR method described above. The DNA content in this case was markedly lower than with the bivalent vaccine samples, but still exceeded the EMA limit by a factor of 18-70 [8].

4. Risk assessment​

We must assume that the recombinant DNA found in the mRNA vaccines can be introduced into the cells of our body, and that this will be aided by the lipid nanoparticles, just as is the case with the mRNA itself. This poses several different kinds of health risks.

4.1. Extended duration of spike protein expression​

One key argument that is regularly used in order to promote the perception of mRNA vaccine safety is that mRNA is short-lived in vivo, and that the expression of the encoded antigen will therefore be of short duration also. For example, the EMA assessment report on the Pfizer vaccine states, with respect to animal experiments on a model vaccine that were accepted en lieu of proper studies on the actual COVID-19 vaccine [4, p. 46]:

As expected with an mRNA product, the luciferase expression was transient … The signal decreased slowly during the first 72 hours and after 6 and 9 days the signals were further weakened to approximately levels of 18 and 7 times the signals obtained from animals injected with buffer control.
These findings seem to agree with two in vitro studies that compared the duration of protein expression between messenger RNA species, which were identical in sequence but contained uridine or mψU, respectively; as noted above, the latter is also present in the Pfizer and Moderna mRNA vaccines. In both studies [1,10], the mψU-modified RNA species induced significantly higher levels of protein expression, yet nevertheless this elevated expression decreased with a half-life similar to that of the unmodified RNA. None of the half-lives which can be inferred from the data in either study exceeds 4.5 days.

It is, however, clear from multiple studies on vaccinated individuals that both the spike protein itself and nucleic acids encoding it can be detected, in the bloodstream and in various organs, for weeks and even months after the injection [11–15]. This discrepancy between in vitro and in vivo studies has so far been difficult to understand. The high levels of residual plasmid DNA in the vaccines detected by McKernan now suggest a plausible explanation.

For the bacterial plasmid DNA to support prolonged expression of the spike protein, two conditions must be fulfilled:

  1. the plasmid DNA must persist inside our body cells, and
  2. the spike protein gene on that plasmid must be transcribed into mRNA by our own cellular RNA polymerase II.
While we do not yet have any direct experimental data on Pfizer’s and Moderna’s spike expression plasmids, precedent suggests that indeed both of these requirements are met. Recombinant plasmids expressing coagulation factor IX have been found to persist in the liver cells of experimental animals at stable levels for up to 1.5 years [16,17], which was the entire duration of the experiment. One might object that the plasmids used in these studies were circular, whereas most of the plasmid DNA contained in the mRNA vaccines is probably in the linear form (see Section 1.2). In response, we note that, firstly, some circular plasmid DNA likely remains (see Section 3.2.1), and secondly that recombinant viral DNA has been shown to persist in linear form within animals for equally long periods of time [18], which suggests that the same can occur with plasmid DNA.

In the cited studies [16,17], the gene encoding the protein of interest (factor IX) had been under the control of a mammalian promoter, and indeed the factor IX protein was expressed at stable levels throughout. In contrast, the spike protein gene contained in Pfizer’s and Moderna’s expression plasmids is under the control of a T7 bacteriophage promoter. We cannot assume a priori that this promoter will function in the absence of its cognate T7 RNA polymerase. It has, however, been experimentally confirmed that indeed the T7 promoter also binds the cellular RNA polymerase II and causes protein expression in mammalian cells [19].

In summary, the possibility that the observed long-lasting expression of spike protein is caused by the plasmid DNA contained in the mRNA vaccines must be taken seriously. Prolonged persistence of spike protein mRNA and its expression after vaccination, detected in biopsies and autopsies, has been unambiguously linked to serious harm [14,20], which is most likely mediated by immune attack on the cells expressing this foreign antigen. The omission of the corresponding experimental studies at the stage of preclinical trials, in conjunction with the scale of this contamination, creates an altogether unacceptable safety risk.

4.2. Risks associated with SV40-derived regulatory DNA sequences​

One feature that was identified by McKernan on Pfizer’s but not on Moderna’s expression plasmids [6] is a promoter derived from the SV40 virus, which belongs to the polyoma family (see Section 4.2). This promoter is located upstream of the kanamycin resistance gene; and since it is active in mammalian cells, the protein encoded by this resistance gene will be expressed in any cell containing this DNA. Like the spike protein, this protein is a foreign antigen, and it, too, may therefore trigger an immune attack on the cells expressing it.

The SV40 promoter also includes an internal origin of replication that can potentially cause copies of the plasmid to be made inside mammalian cells [21]. This will require the presence of the viral large T antigen, a protein which directly recognizes this origin and then initiates the replication of the DNA molecule. This protein is not encoded by the plasmid, nor is it normally present in our body cells, but it might be supplied by either the SV40 virus itself or by a related polyoma virus. A minority of the human population is latently infected with SV40, and such latent infection is associated with some malignant and non-malignant diseases [22]. Should a copy of the Pfizer plasmid be taken up into a cell harboring SV40, then additional copies of it might indeed be formed.

Two related polyoma viruses that are much more widespread in the human population are the BK and the JC virus [23,24]. The JC large T antigen is apparently less effective in conjunction with the SV40 origin than is SV40’s own protein [25], but the replication of Pfizer’s plasmid in cells latently infected with JC or BK viruses can nevertheless not be ruled out. Additional copies of the plasmid generated in this manner would amplify all of the other risks discussed in this section, with the possible exception of non-specific inflammation (see Section 4.4).

4.3. Genomic insertion of the plasmid DNA​

The scenarios discussed so far all involve the independent episomal persistence of the plasmid DNA; it will be present near the chromosomes (within in the cell nucleus), but it will not have become an integral part of any of them. Such independent, non-replicating plasmid molecules will tend to be lost during cell division [26]. However, as we will see, in some cases a plasmid molecule may indeed be integrated into one of its host cell’s chromosomes, and it will then be inherited by all descendants of that cell.

Chromosomal integration is one form of “genotoxicity”, i.e. toxicity which causes genetic damage. With respect to the possibility of such effects, the EMA assessment report on the Pfizer mRNA vaccine notes succinctly [4, p. 50]:

No genotoxicity studies have been provided. This is acceptable as the components of the vaccine formulation are lipids and RNA that are not expected to have genotoxic potential.
Apparently, EMA’s experts were assuming that RNA in general will not affect the integrity of the host cell genome. This view is incorrect, and the first evidence to prove it so has recently celebrated its fiftieth anniversary [27]. However, the detection of copious amounts of plasmid DNA in both manufacturers’ vaccines now obviates the need to make that case. Surely even EMA’s scientists will be aware that this DNA may be integrated into the genome of human host cells. No specific sequence features are necessary for such integration to occur, and accordingly it has been observed alike with the DNA of mammalian viruses, bacteriophages, and plasmids [28]. It is worth noting that such insertions can occur in random locations of the genome, but genes which are being actively expressed by the cell are more commonly affected [29].

The stable chromosomal integration of a bacterial plasmid into the chromosomal DNA of mammalian cells was demonstrated as early as 1982 [30]. The plasmid in question shares multiple features with those used in the production of Moderna’s and Pfizer’s mRNA vaccines. The introduction of foreign or modified genes into mammalian cells using this and similar techniques has since become commonplace in experimental research and in biotechnology. The methodology is referred to as transfection, and organisms modified in this manner as transgenic. We note that stable integration can occur with both linear and circular plasmid DNA [31].

In this context, we should also consider the study previously published by Aldén et al. [32], who detected DNA copies of the spike protein gene in a human liver cell line after these cells had been exposed to the Pfizer mRNA vaccine. Based on the assumption that the vaccine contained essentially pure mRNA but not DNA, they took this observation as evidence that the synthetic mRNA had undergone reverse transcription within those cells. Their interpretation is plausible, because such reverse transcription is known to occur in principle, and it has previously been reported in cells of patients infected with the SARS-CoV-2 virus [33]. However, in light of McKernan’s discovery that Pfizer vaccine vials may contain substantial amounts of DNA, it seems equally possible that the observations by Aldén et al. indicated simply the cellular uptake of this DNA. Either way, however, their findings indicate the presence of spike-encoding DNA within those cells, which indicates a risk of genomic insertion.

4.3.1. Genomic insertion in gene therapy using retroviral vectors​

In proper gene therapy, chromosomal integration is often desired since it will correct the gene defect in question in a lasting manner. To this end, special DNA vectors have been developed which have a greatly increased propensity to undergo such integration. These vectors are derived from retroviruses, whose entire survival strategy is based on genomic integration. It turns out, however, that integration, when it occurs at the wrong place within the genome, frequently induces malignant diseases, especially leukemia [34]. This is indeed so common that it has prevented the widespread adoption of gene therapy, even in diseases for which all other therapeutic options are likewise fraught with very grave risks. A good example is adenosine deaminase deficiency, a metabolic disease which wipes out the lymphocytes and thus causes severe combined immune deficiency (SCID), a condition which without treatment is always fatal during infancy. This disease is in principle a very suitable target for gene therapy, yet a bone marrow transplant from a matched and related donor remains the preferred therapeutic option, due to the grave risk of gene therapy-induced malignancies [35].

4.3.2. How does genomic insertion cause malignancies?​

Our genome contains multiple genes which may give rise to cancer if their expression level—the rate at which mRNA and protein molecules are synthesized from them—is either too low or too high. A foreign DNA molecule may perchance insert directly into such a gene and knock it out altogether, or it may insert next to it, and a strong promoter present on that foreign DNA may cause excessive expression of the gene in question. Additionally, it has been observed that insertion events can also cause genome-wide changes in DNA methylation, which will affect the expression levels of many genes; and some of these changes may contribute to the induction of malignancy. Importantly, this effect has been seen not only with viral DNA but also with bacterial plasmids [36].

When cells are isolated from a healthy human or animal organ and grown in cell culture, they will divide for a limited number of generations and then die. In contrast, cells derived from malignant tumors and leukemias can be propagated indefinitely. A similar change may also occur in cultured cells, which thereby become immortalized and typically also lose some features that are characteristic of their tissue of origin. This transformation can be induced e.g. by infecting the cells with the aforementioned SV40 virus. Similarly, the cells can be transformed by transfection with a SV40-derived plasmid which retains the crucial parts of the viral genome, including the gene encoding the large T-antigen. On the other hand, if the large T-antigen is missing from the plasmid, transformation does not typically occur [30]. However, some exceptions have been reported [37,38]. These cases must have arisen from the disruption or dysregulation of cellular genes involved in controlling proliferation.

4.3.3. Genomic integration in germline cells​

Oocytes can be transfected in vivo at certain stages of maturation [39], and so can sperm-producing cells within the testes [40]. In the latter case, the offspring of the animals subjected to such treatment were shown to be transgenic. It can therefore not be ruled out that persons injected with mRNA vaccines that also contain DNA will subsequently give rise to transgenic children. DNA insertion into germline cells might also interfere with early intrauterine development and thereby induce miscarriages or malformations.

4.3.4. How should we assess the risk of genomic insertion?​

It is certainly true that bacterial plasmids have a lower propensity to insert into our chromosomal DNA than do gene therapy vectors especially designed for efficient integration. But exactly how great is the risk in case of the plasmids contained in the mRNA vaccines? The simple answer is that nobody knows. This is not because it is unknowable in principle, but because the appropriate experimental studies in animals, and subsequently in humans, were not done; or if they were, the results have been kept from the public, and apparently also from the regulators.

How would such risks be assessed in properly conducted approval procedures? The current FDA guidance on the testing and approval of gene therapies [41] recommends that, at the clinical testing stage, patients be monitored for a full 15 years after administration, with yearly exams during the initial five years. This applies to vectors with which chromosomal insertion is intended. The guidance document goes on to construct a false dichotomy between inserting and non-inserting vectors, but the dividing line between them remains fuzzy. On the one hand, the guidance suggests that

GT [gene therapy] products that are based on vectors such as plasmids … do not have a propensity to integrate or reactivate following latency, generally present a lower risk of delayed adverse events,
but on the other hand, it states that

changes in the methods used to introduce plasmid DNA vectors into cells … result in higher integration frequencies (Ref. 27).
The reference cited in the latter quote is a study by Wang et al. [42], who unambiguously identified DNA insertion of plasmid DNA in vivo after intramuscular injection, which was followed by electroporation. While electroporation did increase the cellular uptake of the injected DNA relative to the injection of “naked” DNA alone, it was likely much less effective in this regard than the lipid nanoparticles contained in the mRNA vaccines would be. Accordingly, we must expect some extent of chromosomal integration of the contaminating plasmid DNA in vivo.

4.4. Proinflammatory effect of bacterial DNA​

The human innate immune system reacts with inflammation to various bacterial macromolecules, including DNA. The large amounts of DNA present in the vaccines must be assumed to contribute to inflammation near the injection site, and potentially also elsewhere in the body.

5. Conclusion​

The presence of contaminating plasmid DNA in Pfizer’s and Moderna’s mRNA vaccines entails severe health risks, in addition to those which were already known and understood. Preeminent among these risks are the prolonged expression of spike protein, which may lead to correspondingly prolonged and more destructive autoimmune-like inflammation, and the induction of malignant disease after chromosomal integration of the plasmid DNA. Furthermore, the sheer scale of the contamination proves conclusively that the manufacturers have not mastered or properly implemented the designed production processes. Each of these issues alone would be reason enough to demand the immediate withdrawal of these vaccines.

Acknowledgments​

We thank Kevin McKernan and Ulrike Kämmerer for corrections and discussion.

Copyright​

This text is licensed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0). This means that you are free to copy and reuse the content, as long as the original authors are credited. If you make changes to the text, you must explicitly indicate this fact. For details, see the Creative Commons website [43].

References​

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Renewed efforts to inject EU population
7 April 2023


EU sets out on new vaccination campaign and perpetual covid policy
by Thomas Oysmüller

The EU health authority has recommended starting a new vaccination campaign among older people since Wednesday. Austria, as an EU model school, is trying to anchor "pandemic rules" in law for the long term at the same time.

The so-called "pandemic" is over - even by Karl Lauterbach. But vaccination is to continue. The EU health authority even recommends a new Covid vaccination campaign for autumn at the latest. Older people and "risk groups" should get the next booster.

Vaccinate forever

For the ECDC, the "pandemic" is not over either, but only in a different stage. That is how it puts it in the report published on Wednesday, which is supposed to be a "guideline". Now, the "main objectives of COVID-19 vaccination campaigns would continue to be to reduce COVID-19 hospitalisations, severe illness and deaths, and to protect health systems." So there is no backing away from the vaccination narrative at the highest level.

For people aged 80 and over, they even recommend an "immunisation campaign" (the mRNA treatment offers no immunity) in the spring of 2023, which they then claim, using a computer model, would prevent up to 44 per cent of covid hospitalisations. With a "very high vaccination rate".

Austria's Health Minister Johannes Rauch formulated what is currently happening very aptly: It is currently about "integrating the crisis structures into the regular system of the health care system. That clearly sounds like a "permanent pandemic". Rauch says: "The pandemic goes, the virus stays. Once again, Austria seems to be a model pupil for Brussels.

Since Thursday, the "Covid 19 Transfer Act" has been under review. It is intended to "permanently anchor proven pandemic rules", writes the health minister. Vaccinations, tests, medicines and wastewater monitoring are to be "safeguarded". The law is under review until 3 May. Apart from a lot of changes in the epidemic law, the law will ensure that vaccinations can be diligently continued. Provided there are enough poor people.

https://twitter.com/x/status/1644298056064483330
The "Covid 19 Transition Act" has been under review since Thursday. With it, the Ministry of Health wants to transfer "proven pandemic regulations into regular structures". The law is to come into force on 1 July, the day before the "Covid 19 Measures Act" expires.

END OF ARTICLE • TRANSLATED WITH DEEPL


Addition: It was also said in the Corona Investigative Committee, Berlin, No 150 - that BioNTech has acquired a factory in Germany, in order to make their own DNA plasmids, instead of buying them from Pfizer. This means, BioNTech is investing big times into the mRNA platform for many "medical" gene-based interventions and "vaccines" in the near and far future. This does ring a bell together with the aforementioned article and the EU's stubborn persistence to implement supporting laws and new vaccine campaigns.
 
XPan said:
Nevertheless, below you get the professional version of what "Pandora's box" is about, e.g. the extremely high content of bacterial DNA/plasmids in the latest bivalent mRNA jabs, and beyond the already many known paths of illnesses - we face additionally new implications, due to the integration into chromosomes. I also wonder what the additional of antibiotics getting snuck in, might do to all of this...
Click to expand...

Noticed the discussion on SV40 on the quoted section (there is an old video on this somewhere with a lead scientist laughing with colleagues on how it was done - African Green Monkey Kidneys. Can't remember the manufacture (all Stop, just looked it up on SOTT and it was Merck) back then as it relates to Polio in the 50/60's. PubMed provides this look-back:

pubmed.ncbi.nlm.nih.gov

Immunization Safety Review: SV40 Contamination of Polio Vaccine and Cancer - PubMed

Some of the polio vaccine administered from 1955–1963 was contaminated with a virus, called simian virus 40 (SV40). The virus came from the monkey kidney cell cultures used to produce the vaccine. Most, but not all, of the contamination was in the inactivated polio vaccine (IPV). Once the...
pubmed.ncbi.nlm.nih.gov pubmed.ncbi.nlm.nih.gov

Excerpt​

Some of the polio vaccine administered from 1955–1963 was contaminated with a virus, called simian virus 40 (SV40). The virus came from the monkey kidney cell cultures used to produce the vaccine. Most, but not all, of the contamination was in the inactivated polio vaccine (IPV). Once the contamination was recognized, steps were taken to eliminate it from future vaccines.
Click to expand...

Here is the SOTT article transcript:

www.sott.net

Merck vaccine scientist Dr. Maurice Hilleman admitted presence of SV40, AIDS and cancer viruses in vaccines.

One of the most prominent vaccine scientists in the history of the vaccine industry - a Merck scientist - made a recording where he openly admits that vaccines given to Americans were contaminated with leukemia and cancer viruses. In response,...
www.sott.net www.sott.net

snip:
Dr Maurice Hilleman: So what he did, he brought in, I mean we brought in those monkeys, I only had those and this was the solution because those monkeys didn't have the wild viruses but we...

Dr Edward Shorter: Wait, why didn't the greens have the wild viruses since they came from Africa?

Dr Maurice Hilleman: ...because they weren't, they weren't, they weren't being infected in these group holding things with all the other 40 different viruses...
Click to expand...

So, what you cited above is, unless wrong, is cycling back to SV40 in some way. At the time, SV40 caused cancer to proliferate.


4.2. Risks associated with SV40-derived regulatory DNA sequences​

One feature that was identified by McKernan on Pfizer’s but not on Moderna’s expression plasmids [6] is a promoter derived from the SV40 virus, which belongs to the polyoma family (see Section 4.2). This promoter is located upstream of the kanamycin resistance gene; and since it is active in mammalian cells, the protein encoded by this resistance gene will be expressed in any cell containing this DNA. Like the spike protein, this protein is a foreign antigen, and it, too, may therefore trigger an immune attack on the cells expressing it.

The SV40 promoter also includes an internal origin of replication that can potentially cause copies of the plasmid to be made inside mammalian cells [21]. This will require the presence of the viral large T antigen, a protein which directly recognizes this origin and then initiates the replication of the DNA molecule. This protein is not encoded by the plasmid, nor is it normally present in our body cells, but it might be supplied by either the SV40 virus itself or by a related polyoma virus. A minority of the human population is latently infected with SV40, and such latent infection is associated with some malignant and non-malignant diseases [22]. Should a copy of the Pfizer plasmid be taken up into a cell harboring SV40, then additional copies of it might indeed be formed.

Two related polyoma viruses that are much more widespread in the human population are the BK and the JC virus [23,24]. The JC large T antigen is apparently less effective in conjunction with the SV40 origin than is SV40’s own protein [25], but the replication of Pfizer’s plasmid in cells latently infected with JC or BK viruses can nevertheless not be ruled out. Additional copies of the plasmid generated in this manner would amplify all of the other risks discussed in this section, with the possible exception of non-specific inflammation (see Section 4.4).
Click to expand...
 
dredger said:
Another speech of Vera Sharav, an holocaust survivor who was already referenced a few times in this thread.
She was contacted by Luxembourgish people to make a statement adressed to the authorities, it's an article in french on a Luxembourg website (.lu) and a short 6m video.
Click to expand...

Mark Crispin Miller looks back at Vera's speech in conjunction to a recent article. So, listened to her speech/film again as a lookback to these last many years.

Being Easter, a number of people called or were overheard to say the mandates are over, the masks are off and the plastic shields are down - it's all in the past now. The inclination was to want to say, you think its over?, yet kept my own council as this road has been traveled before, likely by many here. As Vera says, "never again," which are words that have drifted out of people's minds and they don't want to be reminded, they are not even asking or they never knew.

Miller:
Now, for a good example of the sort of Holocaust denial that Vera blew to smithereens (although it will persist despite its bald stupidity, just like the older, crypto-Nazi sort of Holocaust denial), check out this piece from (where else?) Canada:
Click to expand...

What he is talking of is this CBC article (one can imagine the claptrap in the article - example below):

https://www.cbc.ca/news/canada/otta...-protest-ottawa-conspirary-theories-1.6802958

CBC"
"What's so particularly offensive about this is that they're comparing something like not being able to go to the movies for a few months to things like the mobile killing squads or the gas chambers at Auschwitz."
Click to expand...
:rolleyes:

Miller (and think of the C's "trial run"):
The one that murdered millions throughout Europe was the template for the one now killing billions all around the world, as Vera Sharav cogently explained in Nuremberg
Click to expand...

Vera Sharav’s landmark speech—now illustrated brilliantly by Evan Dominguez and his colleagues at the Press and the Public Project—is here:

Vera Sharav's Historic Nuremberg Speech, Illustrated

(And here’s a version with additional sourcing, provided by Dave Ratcliffe:

Vera Sharav's Historic Nuremberg Speech, Illustrated)
Click to expand...

May you all find some peace in the days to come.
 
#italogate / Italy
13 April 2023

In Italy, the scandals are seemingly mounting. The Corona -investigative Committe Berlin, wrote an article on their homepage "2020 News" wrote an article (by Holger Gräf)

2020news.de

#italogate – Chef der Arzneimittelbehörde exposed - 2020 NEWS

Ein Beitrag von Holger Gräf In Italien geht es derzeit Schlag auf Schlag. Immer mehr ungeheuerliche Fakten kommen ans Licht. Wieder ist es der zum Berlusconi gehörende Sender Mediaset, der in seinem Investigativ-Format „Fuori dal coro“ (zu deutsch: „Über den Tellerrand“) eine neue Bombe platzen...
2020news.de 2020news.de

IMAGE-2023-04-13-143651-696x928.jpg

#italogate - Chief of the (Italian) Medicines Agency exposed
A contribution by Holger Gräf

In Italy, things are going from bad to worse at the moment. More and more outrageous facts are coming to light. Once again, it is the Berlusconi-owned broadcaster Mediaset that has dropped a new bombshell in its investigative format "Fuori dal coro" (in German: beyond horizon of a plate / "Über den Tellerrand").

With undisguised pride, presenter Mario Giordano presents a document dated 18 March 2021 containing proof that the then Director General of the Italian Medicines Agency (AIFA), Dr Nikola Magrini, had personally blocked the investigations into five deaths following the administration of the controversial mRNA "vaccine" substance AstraZeneca. He thus not only neglected the duties that AIFA is legally obliged to perform; he did the exact opposite: he actively prevented the agency from performing those duties.

Dr Magrini, who is considered a close ally of former Italian health minister Roberto Speranza, was secretary-general of the Medicines Agency from 2018 until 17 May 2021.

He had to vacate his seat because voices were growing louder that he might be involved in a scandal involving the AstraZeneca mRNA "vaccine" and deliberately concealing serious side effects, such as blood clots. Officially, however, these reasons were not given as the reason for the change, and both Speranza and Dr Magrini always dismissed accusations of this as wild speculation.

With the revelations of 11 April 2023, the speculation has now become fact, which can no longer be denied. The Mediaset presenter has announced that there will be further revelations.


Mastermind is AstraZeneca

If you are wondering who is ultimately behind all the cover-ups, you finally get an answer: it is the "vaccine" manufacturer itself. In a memo dated 9 March 2021, declared as confidential, the pharmaceutical company suggests that all batches continue to be distributed and administered in Italy, even though deaths have already been reported in other countries.

The background is a suspicious batch of the "vaccine" substance in Austria, which was blocked by the competent authorities because a death had occurred. Another batch was under surveillance.

In Italy, soldier Stefano Paternò dies in Sicily on this day, immediately after being "vaccinated" with AstraZeneca.

But unlike in Austria, AIFA does not withdraw the suspicious batch, but waits for an order to seize and dissect the body from the competent public prosecutor's office. This, however, never happens because Dr Magrini blocked it.

Thus, the entire, probably highly toxic batch is "vaccinated" to an unsuspecting population who, due to a hastily created legal situation, did not even have the opportunity to make an informed, free vaccination decision. During this procedure, all side effects and deaths were deliberately downplayed or even completely concealed. 2020News.de has reported on this.

This new leak in the context of the #italogate scandal shows once again how the pharmaceutical company, in its quest for profit that literally goes over dead bodies, together with a politics that complies with it, proceeded. In the present case, the discontinuation of the entire vaccine was not even in question, only a single batch was to be taken off the market. Actually peanuts for a company like AstraZeneca. And yet they sacrificed human lives rather than dispose of the problem-Change.

END OF ARTICLE • TRANSLATED WITH DEEPL
 
Pierre said:
While even most of the world has cancelled the mandatory vaccine, New York University still request it:

https://twitter.com/x/status/1642664814127218688
Click to expand...
My son went to NYU for a year 10 years ago on a scholarship, I was proud at the time, he came back almost traumatized, got a big dose of woke. Still recovering. Well, pride goes before a fall it seems. Good riddance when you go ivy league, all on the bonfire.
 
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