Now this completely pure speculation on my part so take it with a tiny grain of salt.
This Is Not Medical Advice. This is brain storming.
- Since the "shot" of mRNA needs to be stored at -70 C, that gives me a hint that higher temps has a damaging effect on what is in it. So heating up the area around the injection site to as high a level as is safe to the body may perhaps destroy its desired composition. One way to do this would be to put the cuppings on and then go into a sauna. Russian sauna would be the best as the body will tolerate much higher temperatures if the air is dry. Localizing the heating here I think is the desired way to go as heating the body means faster blood flow !!!! Not good to fight the shot injection.
- Around the injection site inject vitamin C to make the local environment as acidic as possible. Acid has wonderful effect on things, it destroys.
It was interesting to see you post this, since the past couple of days I've been reading research papers on the mRNA vaccines with the aim of finding out how you could potentially damage the protecting lipid nanoparticle (LNP) layer and PEG surrounding the 'payload' (mRNA). I'm not sure how beneficial it would be to destroy/dissolve the LNP layer after being injected but from what I've read 'naked' (unprotected) mRNA in the body will be destroyed by the immune system quite quickly. In the case of being vaccinated I would feel better if I'd know that those 'payloads' will not be able to penetrate my cells.
A couple of pages back I was mulling over the idea of destroying the lipid layer with some kind of frequency or 'zapping'. After some research I found out that something like this could maybe be possible with applying some kind of electrical field (since the polarity of the 'package' seems to be of importance for its ability to attach to the cells), but the whole approach seems to complex and unfeasible. Another idea I came up with was to use ultrasound in some way, since in the manufacturing/formation of these NLP structures they actually use sonification with ultrasound (close to how we do our home made liposomal vitamin C). However, so far I haven't found any scientific studies looking at how exactly to use sonification/ultrasound to
destroy the lipids, but ultrasound is mentioned as a way to activate the release of the 'payload' inside the cell (see study below).
In any case, by reading a number of studies I learned a couple of things that may, or may not be useful. If one would want to do this destroying of the LNP, I think key to do this lies in the way the manufacturers have intended the payload to be released once the 'package' is inside the cell. From
this paper we learn that the triggers that will release the payload inside the cell can be
external or
internal.
External triggers:
- Ultrasound (!)
- Heat
- Light
Internal triggers:
- pH
- Enzyme
- Redox (oxidation)
- Hypoxia (oxygen deficiency)
From what I gathered so far another key thing is that the trigger that will work best to 'release the payload'
depends on how the LNP is constructed and what additional stabilizers (e.g. cholesterol) are used. From the papers on Covid mRNA-vaccines it appears that the chosen trigger for 'payload release' in both Moderna and Pfizer
is the pH level: as the package enters inside of the cell the environment is mor acidic and the drop in pH level will dissolve the protecting LNP layer, and thus releasing the payload (mRNA code). The triggering pH level according to a couple of studies is ca 4.5 (the pH level drops from ca 7 [neutral]).
As Hi_henry above speculates, would it be possible to locally lower the pH level this low at the injection site? Would a really 'acidic' deltoid muscle dissolve the LNP:s? Is this a completely crazy idea, what do you think?
The other remotely potential and feasible candidates for premature triggering appear to be
ultrasound and
heat. Right now I'm searching and reading papers dealing with the heat aspect. So far, I haven't found anything conclusive (and most papers dealing with this topic are pretty hard to decipher!) but, for instance,
this paper has some figures of how temperatures effect various lipid structures. A cursory glance tells, maybe (!) that if heat would have any effect on the LNP at the injection site it should be at least 40°C, for greater effect close to 80°C.
I don't know if my 'layman's research' on this subject will lead to anything useful but maybe members with proper expertise can follow the leads, if there are any that appear good. I'll past below links to some of the more interesting papers I've read so far, if someone wants to have a look.
An assessment of pH-sensitive cationic lipid nanoparticles for drug delivery
Future considerations for the mRNA-lipid nanoparticle vaccine platform
Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration - Communications Biology
https://cen.acs.org/pharmaceuticals/drug-delivery/Without-lipid-shells-mRNA-vaccines/99/i8
Design and delivery of messenger RNA-based vaccines
https://dundasvalley.files.wordpress.com/2021/08/pfizer-pharmacokinetics-and-toxicity.pdf
Nanomaterial Delivery Systems for mRNA Vaccines
Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration
These three articles were also enlightening:
Ronavax Roulette: Issues with Lipid Nanoparticles (Part One) - Activist Post
mRNA needs to be delivered in lipid nanoparticles, or LNPs, which is why this is the subject of Julie Beal's deep dive into the science.
www.activistpost.com
PEG (polyethylene glycol) is a polymer that’s used in all sorts of things, from spandex and laxatives, to toothpaste and beauty products.
www.activistpost.com