Dr Sabine Stebel:
The S protein was mutated at 6 amino acids.
How this changes the (folding) properties of the protein has not been investigated. It is simply assumed that it will work. There is no justification for the mutations. Which variant is that supposed to be?
Quite apart from that: wrong target. Spike mutates too quickly, nucleocapsid should have been used, this has now also been published, but is ignored.
Dr Sabine Stebel:
As far as the replicase is concerned, the protein that copies the RNA again and again indefinitely, it has been made less toxic to the cell so that it remains in the cell for longer and produces as many copies as possible and thus as much spike protein as possible is produced by the cell, because (as is well known) a lot does not help much.
No reference to measurements, papers, data. Pure assertions that are not backed up by scientific literature or studies. As if it would be so difficult to put appropriate footnotes. But perhaps there is simply no data?
I am missing:
1) Characterization of the modified replicase compared to the wild type in different human cells. How long does the enzyme remain active, how many copies does it make depending on the cell type. In other words, the usual, boring pharmacokinetics.
2) Expression duration of the spike protein.
3) Characterization of the modified spike protein in comparison to the wild type in different human cells.
4) How well does this modified replicase copy? What is the copying error rate?
5) Is this replicase produced correctly by the cell? What percentage of misfolded enzyme is produced?
6) How is the replicase degraded ?
[metabolized in the body]
7) How does the cell react to this foreign enzyme?
These are just the questions that occur to me spontaneously, without having to think about them at length.
Production as known from modRNA in procedure, also known as #poogate.
One thing is clear:
The product cannot be free of DNA residues from production.
On the one hand, because this is not biologically possible and on the other, because we now know this from painful, experimental experience.
The same dirty production process is used as BioNTech, which has not even been evaluated in comparison to PCR production. What could possibly go wrong?
how are we going to protect ourselves?
We know there are protocols of dissolving that chip crap inside, but still…
