Nicola studied this unnatural CGG CGG. I leave you a thread about how all the pieces were assembled.
Nicola does it again!!!
ZERO PATIENT DISCOVERED!!!
@BidoliNicola
And with this piece you have helped me to have A MORE COMPLETE PUZZLE
.
Let's see it
I TRANSLATE from Nicola: "In this thread of mine, you can read the codename of the first real patient "zero": 14XN611.
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used by the PI of the GOF project in Wuhan, Daszak. 14XN611 was obtained from 2 "historical" coronavirus samples, deposited at WIV (WIV1 and WIV16) to create a clone on which to graft the membrane fusion between a HCOV / HKUI coronavirus and human patient 0 cells."
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Bat SL-CoVs WIV1 and WIV16 are the closest relatives of SARS-CoV. These 2 viruses are identical in genomic structure except for an additional ORF (ORFX) with no homology to another known protein sequence.
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So I just had to follow that codename 14XN611 to look for the paper that talked about those OrfX trials. And here it is
Authors: P. Daszak and... S. Zhengli
Did anyone doubt that they worked together and WHO had not been objective?
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Well, just in case there are any doubts. This study concludes that there is a protein in a reading frame not found so far in other genomes. It is OrfX, responsible for modulating the immune response in the host. It inhibits IFN and activates NF-kB (vascular inflammation
).
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And this is important: these experiments using live viruses were conducted under biosafety level 2 (BSL2)
conditions.
The research conducted by Baric was carried out in laboratories of safety levels 3 and 4 and with maximum protective measures.
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BUT experiments carried out in China with those same pathogens were judged of "not so much importance" and perhaps due to lack of funding were done in labs
of security level 2!!!
Here an attempt in 2017 to channel that biosafety.
Keep the name Linda Saif
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This caused in 2018-2019 a split between the US and China that was perhaps trying to be channeled into Symposiums organized to address joint protective measures against possible pandemics. Like Keystone2018 with well-known scientists and... the absence of Zhengli! (Wuhan)
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Zhengli was on his own. He had learned enough from Baric to conduct his own gain-of-function trials with transgenic mice. Motive? To create a Panvaccine!
Capable of stopping HIV, SARS, MERS and other threats.
Why did he come to that conclusion?
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Well, because to develop a vaccine that works for many viruses (Panvaccine) it is necessary to introduce many pathogens (in N and E) and manage to generate antibodies for all of them. So in the protein where everything starts, the S, all the spikes of the viruses to be treated will be found.
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Therefore, it was necessary to study the possibilities of this protein in other coronaviruses. And so they did.
Daszak and Zhengli chose a bat coronavirus that could serve as a template. SHC014
And that used the ACE2 receptor for cell entry.
The 2002-3 pandemic caused by severe acute respiratory syndrome coronavirus (SARS-CoV) was one of the most significant public health events in recent history. An ongoing outbreak of Middle East respiratory syndrome coronavirus suggests that this group of viruses remains a key threat and that...
pubmed.ncbi.nlm.nih.gov
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Baric had studied MHV-59 years before. And with this murine virus he could see that the change of residues in the cleavage zone (the furin cleavage) affected virulence. At which positions? 682 and 684!!! The famous PRRAR(R)!
I found it in FCoV when doing WIV04 -FCoV
comparison.
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I leave you with this study. It is the systematic assembly and genetic manipulation of the mouse hepatitis A59 virus genome. The full article.
We have developed a DNA assembly platform that utilizes the nonspecific, highly variable sequence signatures of type IIs restriction enzymes to assemble a full-length molecular clone of murine hepatitis coronavirus (MHV) strain A59. The approach also allows changes...
link.springer.com
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And with the TGEV Baric also studied how to successfully assemble an infectious construction.
A systematic method was developed to assemble functional full-length genomes of large RNA and DNA viruses. Coronaviruses contain the largest single-stranded positive-polarity RNA genome in nature. The approximately 30-kb genome, coupled with regions of genomic instability, has hindered the...
pubmed.ncbi.nlm.nih.gov
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Drosten (he of the PCRS
) had also already studied the different proteins of the 2003 SARS and similarities with other CoV in the 3cl protein.
There is a reason why FCoV is not found in the 3CL protein: it was not until 2004 that its protective effect was discovered.
In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of...
pubmed.ncbi.nlm.nih.gov
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BUT I think this 2016 Wuhan study on FCoV changed the landscape and the "bat lady" had a little light go on when she saw the possibilities of the nsp10 and nsp16 proteins of that virus kept in the background - FCoV, the Pif one.https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC4524257/
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It was the missing piece for effective human cell entry. MHV-59 and TGEV joined it.
And they created a recombinant virus between FIPV (
) and MHV (
) to study how to transfer the infection from one host to another.
And they succeeded
Coronaviruses generally have a narrow host range, infecting one or just a few species. Using targeted RNA recombination, we constructed a mutant of the coronavirus mouse hepatitis virus (MHV) in which the ectodomain of the spike glycoprotein (S) was replaced ...
www.ncbi.nlm.nih.gov
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And from there, despite collaborations and funding to continue studying viruses together, China was already going with a GOF on its own. So a study of FIP cats collected between 2017-2019 in Harbin made them attractive to study in Wuhan.
Feline infectious peritonitis (FIP) is caused by the FIP virus (FIPV), a highly virulent mutant form of feline coronavirus (FCoV). This disease is one…
www.sciencedirect.com
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And in that research they were, in a level 2 laboratory (BSL2) assembling bat viruses with the capacity to infect human cells and in BSL3 studying FCoV virulence in animals. Both with poor measurements.
And the pandemic emerged!!!
And someone was prescient
.